Research Article | Open Access | 10.31586/Biology.0302.05

Proline Formation (produce) in the Bacteria: under KCl stress

  • Hüseyin Kahraman1,* and Cennet Canan Karaderi2
    Department of Biology, Faculty of Art and Science, Inonu University, Turkey
    Department of Biology, Institute of Science, Inonu University, Turkey


Salinity is one of the important abiotic stress affecting microorganism growth and productivity. To survive these stresses most organisms have to stress-adaptation mechanisms. It's one of these things proline. We did not find any similar studies with P. aeruginosa and E. faecalis in our studies. The highest value for proline production at 30 ?C 100 rpm was in E. faecalis 11,074 U/ml and in E. coli 6,833 U/ml. The highest proline production in LB medium containing 37 ?C 100 rpm KCl was found to be in E. faecalis 14,604 U/ml and in E. coli 6,557 U/ml. However, there are studies with E. coli. This experiment revealed that the extracellular proline concentration is proportionally linked to the KCl stress. We should first mention that studies similar to those we were less common in the literature. We did not find any similar studies with P. aeruginosa and E. faecalis in our studies.

Figure 1 of 6


Kaino T, Takagi H. Gene expression profiles and intracellular contents of stress protectants in Saccharomyces cerevisiae under ethanol and sorbitol stresses. Appl Microbiol Biotechnol.2008; 79: 273-83.
Takagi H. Proline as a stress protectant in yeast: physiological functions, metabolic regulations, and biotechnological applications. Appl Microbiol Biotechnol. 2008;81: 211-23.
Brill J, Hoffmann T, Bleisteiner M, Bremer E. Osmotically controlled synthesis of the compatible solute proline is critical for cellular defense of Bacillus subtilis against high osmolarity. J Bacteriol.2011;193(19): 5335-46.
Bayat RA, Ku?vuran ?, Ellialtio?lu ?, ?stun AS. Effects of proline application on antioxidative enzymes activities in the young pumpkin plants (Cucurbita pepo L. and C. moschata Poir.) under Salt Stres. Turk J Agr Nat Sci.2014; 1(1): 25-33.
Masuda M, Takamatu S, Nishimura N, Komatsubara S, et al. Improvement of culture conditions for l-proline production by a recombinant strain of Serratia marcescens. Appl Microbiol Biotechnol.1993;189(43):189-97.
Jensen JVK, Wendisch VF. Ornithine cyclodeaminase-based praline production by Corynebacterium glutamicum. Microbiol Cell Fact.2013; 12: 63-72.
Holmstr?m K, M?ntyl? E, Welin B, Mandal A, et al. Drought tolerance in tobacco. Nature, 1996; 379: 683-84.
Semmler ABT, Whitchurch CB, Mattick JS. A re-examination of twitching motility in Pseudomonas aeruginosa. Microbiol.1999; 145: 2863-73.
Calfee MW, Coleman JP, Pesci EC. Interference with Pseudomonas quinolone signal synthesis inhibits virulence factor expression by Pseudomonas aeruginosa. PNAS, 2001; 98 (20): 11633-37.
Takeuchi S, DiLuzio WR, Weibel DB, Whitesides GM. Controlling the shape of filamentous cells of Escherichia coli. Nano Letters, 2005; 5(9):1819-23.
Kau AL, Martin SM, Lyon W, Hayes E, et al. Enterococcus faecalis tropism for the kidneys in the urinary tract of C57BL/6J mice. Infect Immun.2005; 73(4):2461-68.
Troll W, Lindsley JA. Photometric method for the determination of proline. J Biol Chem. 1954; 215: 655-60.
Sugiura M, Takagi T, Kisumi M. Proline production by regulatory mutants of Serratia marcescens. Appl Microbiol Biotechnol.1985;21: 213-19.
Shibasaki T, Hashimoto S, Mori H, Ozaki A. Construction of a novel hydroxyproline-producing recombinant Escherichia coli by Introducing a proline 4-hydroxylase gene. J Biosci Bioeng. 2000; 90(5): 522-25.
Sugiura M, Suzuki S, Takagi T, Kisumi M. Proline production via the arginine biosynthetic pathway: transfer of regulatory mutations of arginine biosynthesis into a proline-producing strain of Serratia marcescens. Appl Microbiol Biotechnol.1986; 24:153-58.
Csonka LN. Physiological and genetic responses of bacteria to osmotic stress. Microbiol Rev.1989; 53(1):121-147
Article metrics
Views 358
Downloads 245
Citations 0
PDF View Full-Text