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Keywords = Biology

  • Open Access Research Article
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    Trends Journal of Sciences Research 2018, 3(1), 1-9. http://doi.org/10.31586/Biology.0301.01
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    Abstract
    Animals usually use photoperiod as an important environmental cue to time the year. In terms of the winter immunocompetence enhancement hypothesis, animals in the non-tropical zone would actively enhance their immune function to decrease the negative influence of stressors such as low temperature and food shortage in winter. In the
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    Animals usually use photoperiod as an important environmental cue to time the year. In terms of the winter immunocompetence enhancement hypothesis, animals in the non-tropical zone would actively enhance their immune function to decrease the negative influence of stressors such as low temperature and food shortage in winter. In the present study, we mimicked the transition from summer to winter by decreasing photoperiod gradually and examined the variations of immune repsonses in Siberian hamsters (Phodopus sungorus) to test this hypothesis. Twenty two female adult hamsters were randomly divided into the control (12h light: 12h dark, Control, n=11) and the gradually decreasing photoperiod group (Experiment, n=11). In the experiment group, day length was decreased from 12 h: 12 h light-dark cycle to 8 h: 16 h light-dark cycle at the pace of half an hour per week. We found that gradually decreasing photoperiod had no effect on body composition (wet carcass mass, subcutaneous, retroperitoneal, mesenteric and total body fat mass) and the masses of the organs detected such as brain, heart, liver and so on in hamsters. Similarly, immunological parameters including immune organs (thymus and spleen), white blood cells and serum bacteria killing capacity indicative of innate immunity were also not influenced by gradually decreasing photoperiod, which did not support the winter immunocompetence enhancement hypothesis. However, gradually decreasing photoperiod increased phytohaemagglutinin response post-24h of PHA challenge, which supported this hypothesis. There was no correlation between cellular, innate immunity and body fat mass, suggesting that body fat was not the reasons of the changes of cellular immunity. In summary, distinct components of immune system respond to gradually decreasing photoperiod differently in Siberian hamsters.  Full article
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  • Open Access Research Article
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    Trends Journal of Sciences Research 2018, 3(2), 90-95. http://doi.org/10.31586/Biology.0302.05
    14 Views 16 Downloads PDF Full-text (2.105 MB)  HTML Full-text
    Abstract
    Salinity is one of the important abiotic stress affecting microorganism growth and productivity. To survive these stresses most organisms have to stress-adaptation mechanisms. It's one of these things proline. We did not find any similar studies with P. aeruginosa and E. faecalis in our studies. The highest value for proline
    [...] Read more.
    Salinity is one of the important abiotic stress affecting microorganism growth and productivity. To survive these stresses most organisms have to stress-adaptation mechanisms. It's one of these things proline. We did not find any similar studies with P. aeruginosa and E. faecalis in our studies. The highest value for proline production at 30 ?C 100 rpm was in E. faecalis 11,074 U/ml and in E. coli 6,833 U/ml. The highest proline production in LB medium containing 37 ?C 100 rpm KCl was found to be in E. faecalis 14,604 U/ml and in E. coli 6,557 U/ml. However, there are studies with E. coli. This experiment revealed that the extracellular proline concentration is proportionally linked to the KCl stress. We should first mention that studies similar to those we were less common in the literature. We did not find any similar studies with P. aeruginosa and E. faecalis in our studies.  Full article
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    Figure 1 of 6

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  • Open Access Research Article
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    Trends Journal of Sciences Research 2015, 2(3), 90-94. http://doi.org/10.31586/ImageProcesses.0203.02
    45 Views 40 Downloads PDF Full-text (463.181 KB)  HTML Full-text
    Abstract
    In this paper, we present an overview of Morphological Image Processing and edge detection using gradient based on different operators in MATLAB and developed its GUI (Graphical User Interface). This paper describes the basic technological aspects of Digital Image Processing with reference to Morphological techniques used in image processing. The
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    In this paper, we present an overview of Morphological Image Processing and edge detection using gradient based on different operators in MATLAB and developed its GUI (Graphical User Interface). This paper describes the basic technological aspects of Digital Image Processing with reference to Morphological techniques used in image processing. The word morphology commonly denotes a branch of biology that deals with the form and structure of animals and plants. Morphological processing refers to certain operations where an object is hit with a structuring element and thereby reduced to a more revealing shape . Morphology is related to the shapes and digital morphology is a way to describe and analyze the shape of a digital object in image processing. Morphological image processing GUI deals with the detection of X-Ray images and its edge detection process. The complete image processing is done using MATLAB Graphical User Interface Development Environment (GUIDE).  Full article
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    Figure 1 of 4

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  • Open Access Research Article
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    Trends Journal of Sciences Research 2015, 2(4), 134-140. http://doi.org/10.31586/Biology.0204.04
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    Abstract
    Methionine, a sulfur amino acid, is the first amino acid that is required for many proteins, during synthesis. Our preliminary studies showed that this compound was produced during the late (post-stationary) secondary phase of growth. Therefore, restriction of methionine may be a useful strategy in limiting cancer growth. The bacterial
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    Methionine, a sulfur amino acid, is the first amino acid that is required for many proteins, during synthesis. Our preliminary studies showed that this compound was produced during the late (post-stationary) secondary phase of growth. Therefore, restriction of methionine may be a useful strategy in limiting cancer growth. The bacterial strain used in this study was Citrobacter freundii (NRRL B-2643) and their vgb+ recombinant strain. A 1/100 inoculum of overnight cultures grown in LB was made in 50 ml LB in 150 ml Erlenmeyer flasks. Inocula in flasks were grown for 24 h at 30 ?C in a 200 rpm water-bath. For MGL production, 250 ?L of this O/N culture was then inoculated into 150 mL conical flask containing 50 mL of sterile mineral salts medium supplemented with 1 % or 0.1 % (w/v) glucose, respectively. This was incubated for 96 h at 30 ?C, 200 rpm on an orbital shaker. The highest MGL concentration (2,02) was reached by the recombinant strain of Cf[pUC8:15] 72 h after the start of incubation MM+0,1% glucose source. In comparison, the wild type strain produced 3,14 of MGL concentration 72 h was reached MM+0,1% glucose source. The poor media and secondary phase (72 h and up) was used to for MGL production. This is more appropriate. Plasmid is disadvantages in the secondary stage.  Full article
    Figures

    Figure 1 of 5

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