Research Article | Open Access | 10.31586/Biochemistry.0304.02

The Modulatory Activity of Justicia carnea in Plasmodium Infected Mice


The study investigated the antiplasmodial potency of Justicia carnea in plasmodium infected mice. The aqueous leaf extract of Justicia carnea was subjected to phytochemical screening using GC-FID. The suppressive, prophylactic and curative activity of the studied plant was accessed after oral administration of standard drugs (5mgkg-1 choroquine and 4mgkg-1 artesunate) and 400-800mgkg-1 of the extract. The effect of the aqueous leaf extract of the studied plant was also explored in the activities of liver enzymes, oxidative stress markers and hematological indices of plasmodium infected mice using spectrophotomertic methods and a hematology auto analyzer (BC 5300 Mindray England) respectively. The aqueous leaf extract of Justicia carnea showed considerable dose dependent antiplasmodial activity in the suppressive, prophylactic and curative test conducted. The suppressive activity of (92%) of the aqueous leaf extract of the studied plant at 800mgkg-1 was comparable to the standard drugs used while the mean survival time of plasmodium infected mice at 800mgkg-1 was beyond the mean survival time of the infected untreated group. The aqueous leaf extract of the studied plant also exhibited significant improvements in the activities of liver enzymes, oxidative stress markers and some hematological parameters at 800mgkg-1 of the extract when compared with the infected and untreated group. The results of the study exhibited the antiplasmodial activity of the studied plant, nonetheless, the crude extracts of the studied plant can be further purified for synergistic use with other potent antimalarial drugs to combat and achieve total clearance of plasmodium resistant infections.


G. C. Akuodor, G. A. Ajoku, M.N. Ezeunala, K.C. Chilaka, E.C. Asika. Antimalarial potential of the ethanolic leaf extract of Pseudocedrala kotschyi. Journal of Acute Disease (2015) 14:23-27.
M. R. Ibukunoluwa. In vivo anti-plasmodial activity and histopathological analysis of water and ethanol extracts of a polyherbal antimalarial recipe. Journal of Pharmacognosy and Phytotherapy (2017) 9(6):87-100.
O. M. Akanbi, A. A. Omonkhua, C. A. Cyril-Olutayo, R. Y. Fasimoye. The antiplasmodial activity of Anogeissus leiocarpus and its effect on oxidative stress and lipid profile in mice infected with Plasmodium berghei Parasitol. Res. (2012) 110:219-226.
U. C. Njoku, B. A. Amadi, P. U. Amadi. Chemical composition and physicochemical analysis of matured stems of Opuntia dillenii grown in Nigeria. Food Science and Technology (2017) 5(5):106-112.
B. A. Amadi, U. C. Njoku, P. U. Amadi, E. N. Agomuo, O. E. Ezendiokwere, K. T. Nwauche. Assessment of vitamins, protein quality and mineral bioavailability of matured stems of Opuntia dillenii grown in Nigeria. Bioengineering and Bioscience (2017) 5(3):47-54.
E. N. Agomuo, P. U. Amadi, C. U. Ogunka-Nnoka, B. A. Amadi, M. O. Ifeanacho, U. C. Njoku. Characterization of oils from Duranta repens leaf and seed. Oil seeds and crops (2017) 24(6):1-8.
E. P. Dikioye, E. O. Ezendiokwere, U. C. Njoku, M. O. Wegwu. Antimalarial activity of ethanolic leaf extract of Maesobotrya barteri and effects on some hematological and biochemical parameters of mice. Journal of Applied Life Sciences International (2017) 12(4): 1-15.
P. U. Amadi, E. N. Agomuo, U. C. Njoku, M. O. Ifeanacho, J. C. Okereke, C. U. Iheka, J. O. Osuoha. Toxicities of selected medicinal plants and floras of lower phyla.  Alexandria Journal of Medicine (2018) Article in Press
G. M. Correa, F. Antonio, C. Alcantara. Chemical constituents and biological activities of species of Justicia – A review. Brazilian Journal of Pharmacognosy (2011) 22 (1):220-238.
S. Badami, R. Aneesh, S. Sankar, M. N. Sathishkumar, B. Suresh, S. Rajan. Antifertility activity of Derris brevipes variety coriacea. J Ethnopharmacol (2003) 84: 99-104.
D. S. S. Kpoviéss, F. Gbaguidi, J. Gbénoua, G. Accrombessi, M. Moudachiroua, E. Rozet, P. Hubert, J. Quetin-Leclercq. Validation of a method for the determination of sterols and triterpenes in the aerial part of Justicia anselliana (Nees) T. Anders by capillary gas chromatography. J Pharmaceut Biomed, (2008) 48: 1127-1135.
D. A. Lorke. New approach for acute toxicity testing. Arch Toxicol (1983) 54: 275-287.
NIH. Guide for the car and use of Laboratory animal (Revised) Washington: NIH Publication; (1985) p. 83-23.
J. F. Riley, W. Peters. The antimalarial activity of some quinolone esters. American Tropical Medical Parasitology (1970) 84:209-222.
J. E. Okokon , E. O. Ettebong , J. A. Udobang, J. Obot. Antiplasmodial and antiulcer activities of Melanthera scandens. Asian Pacific Journal of Tropical Biomedicine (2012) 2:16-20.
B. Adzu, O. A. Salawu. Screening Diospyros mespiliformis extract for antimalaria potency. Int J Biol Chem Sci (2009) 3(2): 271-276.
S. Reitman, A. A. Frankel. Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology. (1957) 28 (1): 56–63.
P. J. Write, P. D. Leathwood, D. T. Plummer. Enzymes in rat urine. Alkaline phosphatase. Enzymology (1972) 42:31– 42.
O. G. Owen. Determination of glutathione and glutathione disulfide using glutathione reductase and 2- vinylpyridine. Anal Biochem, (1980) 106: 207-212.
J. A. Woolliams, G. Wiener, P. H. Anderson, C. H. McMurray. Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of copper in the blood in various breed crosses of sheep. Res Vet Sci, (1983) 34:69—77.
I. Todorova, G. Simeonova, D. Kyuchukova, D. Dinev, V. Gadjeva.  Reference values of oxidative stress parameters (MDA, SOD, CAT) in dogs and cats.  Comp. Clin. Path.  (2005) 13 190–194.
H. Shigemori, T. Kagata, H. Ishiyama. New monoterpene alkaloids from Nauclea latifolia. Chem. Pharm. Bull. (2003) 51:58-61
B. M. Matur, T. Mathew, C. I. C. Ifeanyi. Analysis of the phytochemical and in vivo antimalarial properties of Phyllanthus fraternus Webster extract. New York Sci J (2009) 2: 12-19.
K. C. Lui. Antimalarial activity of artemisinin annua flavonoid from whole plants cell culture. Plant cell (1992) pp. 637-670.
A. A. Sultan, V. Thathy, U. Frevert, et al. TRAP Is Necessary for Gliding Motility and Infectivity of Plasmodium Sporozoites. Cell (1997) 90(3):511–522.
A. M. Lisewki, J. P. Quiros, A. K. Adikejavan, A. K. Eastmant, et al. Supergenomic network compression and the discovery of EXP1 as a glutathione transferase inhibited by artesunate. Cell (2014) 158 (4):916-28.
P. Viriyavejakul, V. Khachonsaksumet, C. Punsawad. Liver changes in severe Plasmodium falciparium malaria: histopathology, apoptosis and nuclear factor Kappa B expression. Malaria journal (2014) 3:106
A. Akhere, O. Mojisola, C. Cyril-Olutayo, M. Olusegun, O. A. Adebay. Antimalarial, hematological, and antioxidant effects of methanolic extract of Terminalia avicennioides in Plasmodium berghei-infected mice. Parasitol Res (2013) 112:3497 – 3503.
E. E. Asangha, G. O. Igile, I. A. Iwara , P. E. Ebong, O. A. Eseyin. Hematological Indices of Plasmodium berghei Infected Mice Treated with ethanol extract and fractions of Nauclea latifolia roots. Int.J.Curr.Microbiol.App.Sci (2017) 6(12): 2546-2556.
Y. A. Kabiru, A. Abdulkadir, A. Timothy, U. M. Gbodi, et al. Evaluation of haematological changes in Plasmodium-berghei-infected mice administered with aqueous extract of Phyllantus amarus. Pakistan Journal of Biological Sciences, (2013) 16: 510-516.
S. Percario, D. R. Morreira, B. Q. Gomes, A. M. Ferreira. Oxidative stress in malaria. Int J. Mol. Sci,. (2012) 13(12):16346-16372.
G. Karupiah, N. G. Hunt, N. J. King, G. Chaudhri, G. NADPH oxidase, Nrampl and nitric oxide synthase in the host anti-malaria response. Rev. Immunogent, (2000) 2:387-415.
M. Kurata, M. Suzuki, N. S. Agar. Antioxidant systems and erythrocyte life-span in mammals. Comp Biochem. Phys. B, (1993) 106:477-487.


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November 20, 2018
How to Cite
S. CHIDI, Alozie et al. The Modulatory Activity of Justicia carnea in Plasmodium Infected Mice. Trends Journal of Sciences Research, [S.l.], v. 3, n. 4, p. 151-160, nov. 2018. ISSN 2377-8083. Available at: <>. Date accessed: 21 apr. 2019.